SOD1, a New Kluyveromyces lactis Helper Gene for Heterologous Protein Secretion
نویسندگان
چکیده
منابع مشابه
SOD1, a new Kluyveromyces lactis helper gene for heterologous protein secretion.
Bottlenecks in protein expression and secretion often limit the development of industrial processes. By manipulating chaperone and foldase levels, improvements in yeast secretion were found for a number of proteins. Recently, sustained endoplasmic reticulum stress, occurring due to recombinant protein production, was reported to cause oxidative stress in yeast. Saccharomyces cerevisiae cells ar...
متن کاملKluyveromyces lactis gene KlBIM1 in high copy state suppresses heterologous protein secretion
2 Vilnius University, Department of Botany and Genetics, Vilnius, Lithuania The aim of this work was to investigate the genes that under over-expression in the yeast Kluyveromyces lactis decrease the yield of secretory proteins. The MD2/1-9 mutant strain with an enhanced ability to secrete different proteins was used. The genes suppressing the protein secretion process were detected by using th...
متن کاملInducible amplification of gene copy number and heterologous protein production in the yeast Kluyveromyces lactis.
Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid recombinase gene. We increased the production and secretion of two heterologo...
متن کاملComplete Genome Sequence of Kluyveromyces lactis Strain GG799, a Common Yeast Host for Heterologous Protein Expression
We report the genome sequence of the dairy yeast Kluyveromyces lactis strain GG799 obtained using the Pacific Biosciences RS II platform. K. lactis strain GG799 is a common host for the expression of proteins at both laboratory and industrial scales.
متن کاملHeterologous products from the yeast Kluyveromyces lactis: exploitation of KlPDC1, a single-gene based system
Heterologous products are frequent applications of microbial technology. Depending on the product, the host-vector system has to be chosen in order to fulfill process requirements. This short review summarizes published and unpublished results obtained for the production of heterologous metabolites and proteins from a single host, the yeast Kluyveromyces lactis, and by means of tools – promoter...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00955-08